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Literature summary for 2.4.3.1 extracted from

  • Paulson, J.C.; Beranek, W.E.; Hill, R.L.
    Purification of a sialyltransferase from bovine colostrum by affinity chromatography on CDP-agarose (1977), J. Biol. Chem., 252, 2356-2362.
    View publication on PubMed
Search for more literature for 2.4.3.1

General Stability

General Stability Organism
glycerol, 50%, stabilizes during storage Bos taurus
high protein concentrations stabilize Bos taurus
use of plastic containers instead of glass stabilizes Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Bos taurus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41500
-
 1 * 41500, low molecular weight form, SDS-PAGE under non-reducing conditions Bos taurus
42900
-
 low molecular weight form, sedimentation equilibrium centrifugation, two enzyme forms differing in molecular weight when submitted to gel filtration, the smaller one is probably a degradation product of the larger one Bos taurus
44000
-
 1 * 44000, low molecular weight form, SDS-PAGE under reducing conditions Bos taurus
45000
-
 low molecular weight form, gel filtration, two enzyme forms differing in molecular weight when submitted to gel filtration, the smaller one is probably a degradation product of the larger one Bos taurus
53500
-
 1 * 53500, high molecular weight form, SDS-PAGE under non-reducing conditions Bos taurus
56000
-
 1 * 56000, high molecular weight form, SDS-PAGE under reducing conditions Bos taurus
57900
-
 high molecular weight form, sedimentation equilibrium centrifugation, two enzyme forms differing in molecular weight when submitted to gel filtration, the smaller one is probably a degradation product of the larger one Bos taurus
80000
-
 high molecular weight form, gel filtration, two enzyme forms differing in molecular weight when submitted to gel filtration, the smaller one is probably a degradation product of the larger one Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
CDP-hexanolamine-agarose affinity chromatography Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
colostrum
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26 28 purified enzyme Bos taurus

Storage Stability

Storage Stability Organism
-20°C, in 50% glycerol, 12 mM cacodylate, pH 5.3, 2 months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-N-acetylneuraminate + asialo-orosomucoid
-
 Bos taurus CMP + orosomucoid
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R sialic acid is linked to C-6 of the galactose residue Bos taurus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 44000, low molecular weight form, SDS-PAGE under reducing conditions Bos taurus
monomer 1 * 53500, high molecular weight form, SDS-PAGE under non-reducing conditions Bos taurus
monomer 1 * 41500, low molecular weight form, SDS-PAGE under non-reducing conditions Bos taurus
monomer 1 * 56000, high molecular weight form, SDS-PAGE under reducing conditions Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.2 5.5 most stable Bos taurus